# Import demultiplexed joined reads (R1+R2 joined)

**URL:** https://forum.qiime2.org/t/import-demultiplexed-joined-reads-r1-r2-joined/1356
**Category:** User Support
**Tags:** dada2, joining, paired-end, import
**Created:** [September 29, 2017, 7:50pm UTC](https://forum.qiime2.org/t/import-demultiplexed-joined-reads-r1-r2-joined/1356 "2017-09-29T19:50:49Z")
**Posts on this page:** 4
**Page:** 1

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### Author: ![MajdaD](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/m/d2c977/32.png) [@MajdaD](https://forum.qiime2.org/u/MajdaD)
#### Post date: [September 29, 2017, 7:50pm UTC](https://forum.qiime2.org/t/import-demultiplexed-joined-reads-r1-r2-joined/1356/1 "2017-09-29T19:50:49Z")

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Dear colleagues,

I am a new Qiime 2 user and I am attempting to import joined fastq files (this means that I have only one fastq per sample that includes R1+R2 joined). I am not sure how to import it into Qiime 2 in order to do quality filtering with DADA2.

Thank you!

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### Author: ![ebolyen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ebolyen/32/11_2.png) [@ebolyen](https://forum.qiime2.org/u/ebolyen)
#### Post date: [September 29, 2017, 8:54pm UTC](https://forum.qiime2.org/t/import-demultiplexed-joined-reads-r1-r2-joined/1356/3 "2017-09-29T20:54:27Z")

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Hey @MajdaD,

Ideally you would import them unjoined, and allow DADA2 to do QC on both directions before merging. [This thread](https://forum.qiime2.org/t/denoise-single-error-associated-with-p-trunc-len-values/1130) has some good discussion on why you'd be better off doing that.

If that isn't possible, you could probably treat them as single-end so long as your read-joiner produces "good" quality scores for the overlapped region between R1 and R2 (see linked thread for discussion), you could use `denoise-single`.

If that also isn't the case, `deblur` should work fine on your merged reads, as it works in a different way.

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [October 31, 2017, 3:14am UTC](https://forum.qiime2.org/t/import-demultiplexed-joined-reads-r1-r2-joined/1356/5 "2017-10-31T03:14:05Z")

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This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.

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### Author: ![jairideout](https://forum.qiime2.org/user_avatar/forum.qiime2.org/jairideout/32/9_2.png) [@jairideout](https://forum.qiime2.org/u/jairideout)
#### Post date: [November 30, 2017, 6:21pm UTC](https://forum.qiime2.org/t/import-demultiplexed-joined-reads-r1-r2-joined/1356/6 "2017-11-30T18:21:33Z")

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The QIIME 2 2017.11 release has expanded support for analyzing paired end reads! See the [paired end reads community tutorial](https://forum.qiime2.org/t/analyzing-paired-end-reads-in-qiime-2/2021) for more details. 🎉
