Hi Users, I have qiime2-2019.4 installed through miniconda in Linux Mint 19.1. I have sequences bacterial V3-V4 regions. The outsourcing company provided me forward and reverse sequences as fastq.gz files and primer and barcodes as text (provided at the end). I also asked them for barcodes as fast…

Hi @ashisroybarman , Welcome to the forum! Thank you for providing the details of your problem, very helpful for troubleshooting. Can you clarify one more thing for us, do you have a single fastq file for all of your forward and reverse files (multiplexed) or do you have a forward and reverse file…

Hi Mehrbod_Estaki, Many thanks for your quick reply and sorry for being late in replying. I have a forward and a reverse file for every sample, all together 14 files for 7 soil samples. As you have mentioned, I am new and not sure whether these were demultiplexed. I want to perform analysis and c…

I have also tried importing with the tutorial "Importing data>Casava 1.8 paired-end demultiplexed fastq" (URL: Importing data — QIIME 2 2019.7.0 documentation ) for my set of R1 and R2, it generated following error: "There was a problem importing casava-18-paired-end-demultiplexed: Missing one or m…

Hi @ashisroybarman . Since you have a separate forward and reverse file for each sample, these reads have already been demultiplexed, and from the looks of your reads the facility has already removed primers and barcodes from your reads. They have left the barcodes on line 1 of your reads files for …

Hi Mehrbod_Estaki, Thank you for your valuable suggestions.

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Illumina data import with sequence as fastq.gz, primer and barcodes as txt files

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