Illumina data import with sequence as fastq.gz, primer and barcodes as txt files

User Support
1

Hi Users,
I have qiime2-2019.4 installed through miniconda in Linux Mint 19.1.
I have sequences bacterial V3-V4 regions. The outsourcing company provided me forward and reverse sequences as fastq.gz files and primer and barcodes as text (provided at the end). I also asked them for barcodes as fastq.gz format, however, they are reluctant to provide me. I am novice in this and unable to move a step following the provided importing techniques (Moving Picture or FMT).

I require your kind support regarding:

  1. How to import
  2. Following workflow such as whether or how to remove barcodes and primer sequences etc.

Waiting for your kind response…

Less view of forward:

@700823F:460:HTJ2MBCX2:2:1101:4501:2076 1:N:0:GAGATTCC+GTACTGAC
ACGCCGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACAAGAGTAACTGCTTGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTT
+
<<.<GAGGIIIIIIIIIGGIIIIIIIIIIIIIIIIIIGIIIIIIIIIIIIIIIIIIIIIIIIIIGGGGGGGIGIIIGGIIGIGIGGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGIIIIIIIGGGGGIIGIIIIIIIIGIIIGIIGIIIGIIIGIIGIGGIIIIIIGIIIIIGIIIIGGGGIGIIIIIIIIIIIIGGGGGGGGIIIIIGGIGGIIGIGGGIIIIIIGIIIIIIIGGGGGGIIGG
@700823F:460:HTJ2MBCX2:2:1101:6407:2205 1:N:0:GAGATTCC+GTACTGAC
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATCATACTTTAGTAAGCTGCCTTCGCAATTGGTGTTCTGTGACATATCTATGCATTTCACCGCTACTTGTCACATTCCGCCTACCTCAAGTACATTCAAGCCTATCAGTATCAAAGGCACTGCGATAGTTAAGCTACCGTCTTTCACCCCTGACTTAATAGGCCGCCTACGCACCCTTTAAACCCAATAAATCCG
+
GGGGGGAGGGIIIIIIGGIIGIIIIIGGGIGIGGI<GGIIIGGGGIIIIGIGGGGGIIIGGGGIGIGGAGAGGGGGAGGGGGAGGIGGGGGAGGIGGGIIGGGIIIIGGIIIGGIGIIIGIIIGGGGGGIGIIGGIGGGGIGGAGGGIGGAGGAGGGGIGGGIIGGGGGGGGG.AGAGGGGIIIIIGGGGGIIIIIII.GGGGGGGAA.AGAGGGAGGGGGAAGGGGIIAGGGGGGGGI.7GGIIGGAAG

Less view of reverse:
@700823F:460:HTJ2MBCX2:2:1101:4501:2076 1:N:0:GAGATTCC+GTACTGAC
ACGCCGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACAAGAGTAACTGCTTGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTT
+
<<.<GAGGIIIIIIIIIGGIIIIIIIIIIIIIIIIIIGIIIIIIIIIIIIIIIIIIIIIIIIIIGGGGGGGIGIIIGGIIGIGIGGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGIIIIIIIGGGGGIIGIIIIIIIIGIIIGIIGIIIGIIIGIIGIGGIIIIIIGIIIIIGIIIIGGGGIGIIIIIIIIIIIIGGGGGGGGIIIIIGGIGGIIGIGGGIIIIIIGIIIIIIIGGGGGGIIGG
@700823F:460:HTJ2MBCX2:2:1101:6407:2205 1:N:0:GAGATTCC+GTACTGAC
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATCATACTTTAGTAAGCTGCCTTCGCAATTGGTGTTCTGTGACATATCTATGCATTTCACCGCTACTTGTCACATTCCGCCTACCTCAAGTACATTCAAGCCTATCAGTATCAAAGGCACTGCGATAGTTAAGCTACCGTCTTTCACCCCTGACTTAATAGGCCGCCTACGCACCCTTTAAACCCAATAAATCCG
+
GGGGGGAGGGIIIIIIGGIIGIIIIIGGGIGIGGI<GGIIIGGGGIIIIGIGGGGGIIIGGGGIGIGGAGAGGGGGAGGGGGAGGIGGGGGAGGIGGGIIGGGIIIIGGIIIGGIGIIIGIIIGGGGGGIGIIGGIGGGGIGGAGGGIGGAGGAGGGGIGGGIIGGGGGGGGG.AGAGGGGIIIIIGGGGGIIIIIII.GGGGGGGAA.AGAGGGAGGGGGAAGGGGIIAGGGGGGGGI.7GGIIGGAAG

Sequences of forward, reverse primers and barcodes:
Library id Sample name Index 1 Index 2
LIB32530 YR2-CR2-P1 GAGATTCC GTACTGAC

V3 Forward : CCTACGGGNBGCASCAG
V4 Reverse : GACTACNVGGGTATCTAATCC

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