I want to do some statistical analysis to confirm the changes

Hi all,

I did my microbiome analysis already using QIIME 2 v 2019.1 but now I want to do some statistical analysis to confirm the changes I am seeing in my taxa box plot. However, each group of number is difference (ex. group1 = 10, group2 = 4, group3 =14).
Can I use ANCOM in this situation?
Basically, what I want to know if which changes in the genotype abundances are significantly different or not across my samples.
A would appreciate any suggestion.

Thanks a lot!!
Taichiro

Hi @Taichiro, perhaps our paper is a good starting point to answering your questions. Yes, you can run ANCOM, but it is best to understand the underlying assumption and caveats.

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Many thanks!

Thanks,
Taichiro

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