# how to train my classifier for V3-V4 16SRNA gene region

**URL:** https://forum.qiime2.org/t/how-to-train-my-classifier-for-v3-v4-16srna-gene-region/27020
**Category:** User Support
**Tags:** feature-classifier, rescript
**Created:** [July 10, 2023, 9:18am UTC](https://forum.qiime2.org/t/how-to-train-my-classifier-for-v3-v4-16srna-gene-region/27020 "2023-07-10T09:18:42Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer)
#### Post date: [July 10, 2023, 1:17pm UTC](https://forum.qiime2.org/t/how-to-train-my-classifier-for-v3-v4-16srna-gene-region/27020/2 "2023-07-10T13:17:51Z")

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Hi @Cele_Blua,

> [@Cele\_Blua](#):
>
> my computer crashed when I get the "make our classifier for use on full-length SSU sequences" step

Training the full-length 16S rRNA gene classifier can take a substantial amount of memory. Often requiring anywhere from 24 - 64 GB of RAM! You can make use of premade classifiers from the [data resources](https://docs.qiime2.org/2023.5/data-resources/) page. However, if you do not have the memory to train the classifier, there is a chance you might not have enough memory to use the classifier, at least without modifying some options.

> [@Cele\_Blua](#):
>
> I got (nicely) my silva-138.1-ssu-nr99-341f-805r-classifier.qza, but I dont understand how to test it.

You can...

1. simply use the classifier to see if the outputs make sense.
2. use some of the built-in [evaluation](https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494/2#heading--seventh-header) functionality of RESCRIPt.

-Cheers!  
-Mike

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