How to merge rep-seqs in qiime ?

[M_F (Meriem)]

HI,

I will use HITDB for classification

qiime feature-classifier classify-sklearn
--i-classifier HITdb_classifier.qza
--i-reads rep-seqs.qza
--o-classification HITdb_taxonomy_qiime2.qza
How can i merge rep-seqs.qza reads for three different samples to run the aforementioned command line ?

[salias (Sergio Alías-Segura)]

Hi @M_F ,

I think you are looking for qiime feature-table merge-seqs.

Does your data come from the same sequencing run? If so, and if you used DADA2, you may want to re-run it with all samples at once, since DADA2 devs recommend to launch a DADA2 run for each sequencing run.

Best,

Sergio