# How to know if my sequence data demultiplexed?

**URL:** https://forum.qiime2.org/t/how-to-know-if-my-sequence-data-demultiplexed/11988
**Category:** User Support
**Created:** [October 16, 2019, 1:16am UTC](https://forum.qiime2.org/t/how-to-know-if-my-sequence-data-demultiplexed/11988 "2019-10-16T01:16:59Z")
**Posts on this page:** 1
**Showing post:** 6

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [October 16, 2019, 3:29am UTC](https://forum.qiime2.org/t/how-to-know-if-my-sequence-data-demultiplexed/11988/6 "2019-10-16T03:29:33Z")

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> [@HebaHussein-1981](#):
>
> no need to do metadata file

You still need a metadata file, you just do not need barcodes.

The sample metadata file contains all sorts of info about the samples, including things like treatment group or other characteristics that you want to use to compare your samples in later steps.

> [@HebaHussein-1981](#):
>
> demultiplexing and can go directly to import manifest for denoising?

Yes! You can import with that manifest file and proceed straight to denoising.

Good luck!

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