I'm so sorry because I've not take a screenshot for my command line. I describe it simply, I am trying to install a metagenome or moshpit platform from April of this year, just for preparing a set of analysis for my new metagenome data, however, I tried many times to install, although the sentences were simple, but the effects were not workable usually. So I tried many times, the wrong messages usually show as my post described and the environment could not be installed. By some unknown reasons, I just successeded twice use the same command with qiime2-moshpit-2025.4. But while I using it dealing data analysis, I found my Ubuntu system of WSL occupy too large storage when the time was passing. So I have to uninstalled the platform, and then I tried to re-install it on my Oracle VirtualBox, because it runs so fast, compared to WSL. As usual, it was difficult to be installed. So I tried to install an older version, for example qiime2-metagenome-2024.10, the problems were as usual, just like my post described. Could you really didn't met errors like me？By the way, I think my Ubuntu Linux environment is completely and originally. Another link about busco discussion is here Failure to download BUSCO DB.