# how to importing data

**URL:** https://forum.qiime2.org/t/how-to-importing-data/26336
**Category:** Technical Support
**Created:** [May 4, 2023, 5:44pm UTC](https://forum.qiime2.org/t/how-to-importing-data/26336 "2023-05-04T17:44:15Z")
**Posts on this page:** 9
**Page:** 1

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### Author: ![baehsung](https://forum.qiime2.org/user_avatar/forum.qiime2.org/baehsung/32/13247_2.png) [@baehsung](https://forum.qiime2.org/u/baehsung)
#### Post date: [May 4, 2023, 5:44pm UTC](https://forum.qiime2.org/t/how-to-importing-data/26336/1 "2023-05-04T17:44:15Z")

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Hi,

I got the FastQ-formatted sequences that were sequenced in the PacBio platform using SMRTbell library. The seqs are demultiplexed. Because barcoded F and R primers amplified one fragment from entire region of interest (~1.0 kb), it does not seem to use the option combining two fragments amplified by F and R direction (R1 and R2) using a paired-end option. I found that the tutorials in qiime2 doc posted several choices showing how to import data for downstream analyses in qiime2 (e.g., Casava 1.8 single-end demultiplexed fastq). Do someone know which one is the choice to import my demultiplexed FastQ data for downstream analyses? If I could importing my data successfully, I think I can move to the next step "seq quality control" using DADA2 that I experienced before.

Thanks.

Hee-Sung

 ![image](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/5/544b7b212b9b92b575016c29b2c34180ef436116.png)

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### Author: ![crusher083](https://forum.qiime2.org/user_avatar/forum.qiime2.org/crusher083/32/9597_2.png) [@crusher083](https://forum.qiime2.org/u/crusher083)
#### Post date: [May 4, 2023, 6:23pm UTC](https://forum.qiime2.org/t/how-to-importing-data/26336/2 "2023-05-04T18:23:13Z")

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Hello,

First of all, I'd like to warn you that your post violates a [Code of Conduct - QIIME 2 Forum](https://forum.qiime2.org/faq#your-work), your work is your work. Requesting someone's code is prohibited. Please, edit it accordingly.  
Second, QIIME 2 does not have plugins for preprocessing PacBio data, but when you obtain a feature table can be you can use QIIME2 downstream with a plethora of analytical methods for tabulated data.

Cheers,  
V

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### Author: ![baehsung](https://forum.qiime2.org/user_avatar/forum.qiime2.org/baehsung/32/13247_2.png) [@baehsung](https://forum.qiime2.org/u/baehsung)
#### Post date: [May 4, 2023, 7:14pm UTC](https://forum.qiime2.org/t/how-to-importing-data/26336/4 "2023-05-04T19:14:08Z")

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Thank you for pointing out this. I edited as recommended by you. Hee-Sung.

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### Author: ![crusher083](https://forum.qiime2.org/user_avatar/forum.qiime2.org/crusher083/32/9597_2.png) [@crusher083](https://forum.qiime2.org/u/crusher083)
#### Post date: [May 4, 2023, 7:53pm UTC](https://forum.qiime2.org/t/how-to-importing-data/26336/6 "2023-05-04T19:53:07Z")

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Hello @baehsung

I apologize, @lizgehret actually pointed out, that you can use q2-dada2 for preprocessing. But it only utilizes information from forward reads.  
In case of paired-end sequencing I would look into official PB software: [GitHub - PacificBiosciences/pbbioconda: PacBio Secondary Analysis Tools on Bioconda. Contains list of PacBio packages available via conda.](https://github.com/PacificBiosciences/pbbioconda)

Cheers,  
V

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### Author: ![gregcaporaso](https://forum.qiime2.org/user_avatar/forum.qiime2.org/gregcaporaso/32/17769_2.png) [@gregcaporaso](https://forum.qiime2.org/u/gregcaporaso)
#### Post date: [May 4, 2023, 8:12pm UTC](https://forum.qiime2.org/t/how-to-importing-data/26336/7 "2023-05-04T20:12:38Z")

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Hi @baehsung and @crusher083,  
I discussed this with some of the other QIME 2 Forum moderators, and we don't agree that this post constituted a code of conduct violation. Rather it seems that there was some a misunderstanding over what you were asking for. I apologize if this created any confusion.

I'm sitting next to @lizgehret right now and she has some additional information that she thinks may be helpful for you. She'll follow up with you on this post.

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### Author: ![lizgehret](https://forum.qiime2.org/user_avatar/forum.qiime2.org/lizgehret/32/17128_2.png) [@lizgehret](https://forum.qiime2.org/u/lizgehret)
#### Post date: [May 4, 2023, 8:18pm UTC](https://forum.qiime2.org/t/how-to-importing-data/26336/8 "2023-05-04T20:18:48Z")

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### Author: ![lizgehret](https://forum.qiime2.org/user_avatar/forum.qiime2.org/lizgehret/32/17128_2.png) [@lizgehret](https://forum.qiime2.org/u/lizgehret)
#### Post date: [May 4, 2023, 9:27pm UTC](https://forum.qiime2.org/t/how-to-importing-data/26336/9 "2023-05-04T21:27:28Z")

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Hi @baehsung,

I wanted to share with you another [forum post](https://forum.qiime2.org/t/importing-analysing-pacbio-hifi-ccs-v1-v9-reads/18319) that provides an example of how you would import your PacBio reads (as you'll need to handle this step prior to dealing with the denoising/dereplicating of your data). Hope this helps - please let us know if you need any further assistance!

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### Author: ![lizgehret](https://forum.qiime2.org/user_avatar/forum.qiime2.org/lizgehret/32/17128_2.png) [@lizgehret](https://forum.qiime2.org/u/lizgehret)
#### Post date: [May 4, 2023, 9:27pm UTC](https://forum.qiime2.org/t/how-to-importing-data/26336/10 "2023-05-04T21:27:32Z")

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [June 5, 2023, 3:28am UTC](https://forum.qiime2.org/t/how-to-importing-data/26336/11 "2023-06-05T03:28:07Z")

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