# how to import these reads: Mixed-oriented or not? **URL:** https://forum.qiime2.org/t/how-to-import-these-reads-mixed-oriented-or-not/22685 **Category:** User Support **Tags:** feature-classifier, demux, import, cutadapt **Created:** [April 5, 2022, 11:53am UTC](https://forum.qiime2.org/t/how-to-import-these-reads-mixed-oriented-or-not/22685 "2022-04-05T11:53:51Z") **Posts on this page:** 1 **Showing post:** 7
### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer) #### Post date: [April 12, 2022, 5:28pm UTC](https://forum.qiime2.org/t/how-to-import-these-reads-mixed-oriented-or-not/22685/7 "2022-04-12T17:28:02Z")
Hi @Wieneke, I think you can go ahead and import them as you normally would and then follow these two posts from the same thread. > [@q2-cutadapt output](https://forum.qiime2.org/t/q2-cutadapt-output/17031/24): > > Good news @jessica.song! I think I solved this. fireworks First, thank you for sending me the PDF describing the protocol. I noticed a key statement: pairs of primers (Fw-Rev or Rev-Fw) had to be present in the sequence fragments That is, this particular sequencing facility must expect mixed orientation reads. rotating_light Therefore you need to enter in both primers for each of the --p-front-\* commands (in 5' - 3' orientation). dna Like so: qiime cutadapt trim-paired \ --i-demu… > [@q2-cutadapt output](https://forum.qiime2.org/t/q2-cutadapt-output/17031/28): > > @jessica.song, I forgot to mention, that you'll still need to orient left_right_arrow this output into the same direction prior to any other downstream analyses. You can do this via the [RESCRIPt](https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494) action [orient-seqs](https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494#heading--thirteenth-header). -Good luck! shamrock Let us know if this works. --- _[View the full topic](https://forum.qiime2.org/t/how-to-import-these-reads-mixed-oriented-or-not/22685)._