How to import Roche 454 pyrosequencing data in QIIME2 without using QIIME1?

[colinbrislawn (Colin J Brislawn)]

Hello @Sparkle! Welcome to the forums.

I think the cutadapt plugin will work well on 454 data. Check out this tutorial and see if it looks like a good fit for your data set.

Demultiplexing and Trimming Adapters from Reads with q2-cutadapt

NOTE This tutorial is a work in progress, and is incomplete at the moment. It demonstrates at a high level some of the methods available in the q2-cutadapt plugin available in QIIME 2 2018.2. Please stay tuned here for additional updates as this tutorial is expanded upon in the coming weeks. Multiplexed reads with the barcodes in the sequence reads can be demultiplexed in QIIME 2 using the q2-cutadapt plugin, w…

Colin