# How to import paired end data from miseq?

**URL:** https://forum.qiime2.org/t/how-to-import-paired-end-data-from-miseq/30130
**Category:** User Support
**Tags:** miseq, paired-end, import
**Created:** [May 7, 2024, 11:35am UTC](https://forum.qiime2.org/t/how-to-import-paired-end-data-from-miseq/30130 "2024-05-07T11:35:55Z")
**Posts on this page:** 4
**Page:** 1

<div class="post-metadata">

### Author: ![ehdud0505](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ehdud0505/32/18591_2.png) [@ehdud0505](https://forum.qiime2.org/u/ehdud0505)
#### Post date: [May 7, 2024, 11:35am UTC](https://forum.qiime2.org/t/how-to-import-paired-end-data-from-miseq/30130/1 "2024-05-07T11:35:55Z")

</div>

Hello, everyone.

I'm struggling with data importing right now. I'm using qiime2.2024.2 version.

The data is obtained via miseq and the files are in the form of **OO-forward.fastq.qz, OO-reverse.fastq.qz.**  
Barcode is expected to be exist in the files.

I'm not sure what type to use to import the data into the qiime2.  
I tried Casava, EMP, SampleData, Multiplexed, and Multiplexed and they all give me an error.

When I used iseq before, it existed as a casava type of file name, but this time it's different...

So I tried to create a manifest file and put it in, but it doesn't work.... What should I do?

I'll also upload the manifest file.

I look forward to your response, thank you.

[bioflor-manifest.tsv](https://forum.qiime2.org/uploads/short-url/eBhOWTLT0gZ6ZrgZmBmfQ4ONKQj.tsv) (10.2 KB)

---

<div class="post-metadata">

### Author: ![cherman2](https://forum.qiime2.org/user_avatar/forum.qiime2.org/cherman2/32/17129_2.png) [@cherman2](https://forum.qiime2.org/u/cherman2)
#### Post date: [May 7, 2024, 2:53pm UTC](https://forum.qiime2.org/t/how-to-import-paired-end-data-from-miseq/30130/2 "2024-05-07T14:53:24Z")

</div>

Hi @ehdud0505,  
I think this import guide should be exactly what you want.

[Multiplexed paired-end FASTQ with barcodes in sequence](https://docs.qiime2.org/2024.2/tutorials/importing/#multiplexed-paired-end-fastq-with-barcodes-in-sequence)

Note: you may have to rename your files!

---

<div class="post-metadata">

### Author: ![ehdud0505](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ehdud0505/32/18591_2.png) [@ehdud0505](https://forum.qiime2.org/u/ehdud0505)
#### Post date: [May 8, 2024, 4:54am UTC](https://forum.qiime2.org/t/how-to-import-paired-end-data-from-miseq/30130/3 "2024-05-08T04:54:33Z")

</div>

Hi @cherman2,

The data was successfully imported thanks to your advice to rename the file. Thank you so much!

- Doyoung -

---

<div class="post-metadata">

### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [June 8, 2024, 10:54am UTC](https://forum.qiime2.org/t/how-to-import-paired-end-data-from-miseq/30130/4 "2024-06-08T10:54:40Z")

</div>

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.