I´m working with 16srRNA sequences forward and reverse and i want to import to qiime 2, but i have no barcodes (and i have no chance to get them), surfing on the web, i have found, you can make a manifest document intead of a metadata doc; then i made a manifest one and I wrote the command on qiime2, but it shows an error message that says:
An unexpected error has occurred:
Decoded Phred score is out of range [0, 62].
See above for debug iinfo
what can i do?