How to import forward and reverse seqs?

User Support
1

I´m working with 16srRNA sequences forward and reverse and i want to import to qiime 2, but i have no barcodes (and i have no chance to get them), surfing on the web, i have found, you can make a manifest document intead of a metadata doc; then i made a manifest one and I wrote the command on qiime2, but it shows an error message that says:
An unexpected error has occurred:

Decoded Phred score is out of range [0, 62].

See above for debug iinfo

what can i do?

1 Like
2

Try changing to Phred33. e.g.
qiime tools import
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path manifest.txt
--output-path demux.qza
--input-format PairedEndFastqManifestPhred33V2

3 Likes
3

thanks, that was very useful

6

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