# How to import data from miseq paired end reads 2 x 251

**URL:** https://forum.qiime2.org/t/how-to-import-data-from-miseq-paired-end-reads-2-x-251/2204
**Category:** User Support
**Tags:** pending-development, import, extract-barcodes
**Created:** [December 13, 2017, 4:03pm UTC](https://forum.qiime2.org/t/how-to-import-data-from-miseq-paired-end-reads-2-x-251/2204 "2017-12-13T16:03:04Z")
**Posts on this page:** 1
**Showing post:** 9

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### Author: ![Pseudomonas84](https://forum.qiime2.org/user_avatar/forum.qiime2.org/pseudomonas84/32/629_2.png) [@Pseudomonas84](https://forum.qiime2.org/u/Pseudomonas84)
#### Post date: [December 24, 2017, 6:31pm UTC](https://forum.qiime2.org/t/how-to-import-data-from-miseq-paired-end-reads-2-x-251/2204/9 "2017-12-24T18:31:50Z")

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Hi, thank you very much for your response. I now have a reads.fastq file and a barcode.fastq file. I have already imported the files to qiime2 using the EMPSingleEndSequences type. I tried to do demultiplexing sequences--for sample metadata.tsv, I used the mapping.txt that I have. It's written in the tutorial section to use \*.tsv, but the \*.txt that I've used did not give errors, so I supposed that it is okay? However, when I tried viewing demux.qzv using qiime2 gui so I can decide trim and trun length, I find my graph weird. I must have done something wrong here?

 ![demux qzv](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/1X/1e3a749ea9b573315bfb5a7f1ac925a956361077.png)

My apology if this question may appear stupid--new to qiime/bioinformatics.

Please let me know how to proceed and thank you for your patience.

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