How to handle mixed orientation reads

Hello @ucassee,

This is a great question, and I'm not sure there is an official solution. But have to try because we don't want to lose have the reads!

I had a great discussion about this exact issue, and we discussed the options and what to try first. Let me know if you find this thread helpful: Dealing with non-oriented amplicon libraries

Let me know if you are able to try any of the suggestions in that thread, especially close-ref OTU picking.

Colin

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