### Author:  [@ag1170](https://forum.qiime2.org/u/ag1170)
#### Post date: [November 15, 2023, 8:05pm UTC](https://forum.qiime2.org/t/how-to-extract-sequences-for-specific-samples-in-dataset/28310/1 "2023-11-15T20:05:30Z")
I have looked in the qiime2 forum but no answers have been able to help me yet.
I have 27 samples. I want to extract the rep seqs from one specific sample so I can BLAST them and compare to an isolate I have from this sample's same sampling site. I seem to be repeatedly misunderstanding how I can do this.
So far I have seen these posts:
> [@Error with feature-table filter-seqs: All features were filtered out of the data.](https://forum.qiime2.org/t/error-with-feature-table-filter-seqs-all-features-were-filtered-out-of-the-data/9296):
>
> Dear all, I was trying to run the following command: qiime feature-table filter-seqs \ --i-data allBatches-representativeSeqs.qza \ --m-metadata-file metadata\_allBatches\_full.tsv \ --p-where "organ\_blank IN ('lung', 'blank')" \ --o-filtered-data lungBlank\_allBatches-repSeqs.qza However, I was unable due to an error. Does anyone have an idea of what might be going on? I know that this would not filter all samples out as organ\_blank can take other values. The error message is: Traceb…
> [@How to extract specific sequences per sample?](https://forum.qiime2.org/t/how-to-extract-specific-sequences-per-sample/4073):
>
> Hi, I want to extract specific sequences and check their abundance in different samples/ know from which sample they originate. I have 16S data, and I’m interested in just specific group of microorganisms. So, I would like to extract their sequences in order to create a phylogenetic tree, and be able to link the obtained results with samples metadata. Is that possible? Thanks in advance!
> [@How to get separate sequence fasta file for each sample from feature table after dada2 filtering](https://forum.qiime2.org/t/how-to-get-separate-sequence-fasta-file-for-each-sample-from-feature-table-after-dada2-filtering/9486/4):
>
> Hi @sajjad.sarikhan, Sorry but I'm not sure I quite understand still what you are trying to do. When you say "qiime2 database correspondong to my samples", what exactly are you referring to? If your end goal is to have a separate feature-table, phylogenetic tree, representative-sequences.. etc for each sample instead of the normal combined approach, you can achieve this using a combination of some of the available [filtering methods](https://docs.qiime2.org/2019.1/tutorials/filtering/) after creating the combined version of said files. For examp…
But they either weren't specific enough for what I am trying to do or weren't helpful enough for me (I'm new to QIIME2 btw).
So far I have tried creating a simplified metadata file with only the sample ID of the sample I want to retain, and then I tried using that simplified metadata file in filter-seqs against the OG repseqs from deblur:
qiime feature-table filter-seqs --i-data ../deblur-repseqs.qza --m-metadata-file sample-metadata-dconly.tsv --o-filtered-data dc-onlyseqs
But i received the error:
**Plugin error from feature-table:**
**All features were filtered out of the data.**
**Debug info has been saved to /tmp/qiime2-q2cli-err-a8ngb9bw.log**
I have also tried doing this command where I tried doing filter-samples using my table.qza after deblur (i previously already filtered things like Mitochondria and chloroplasts that I didn't want) with my simplified metadata file and then exported it, but it gave me a .biom file that I can't make any sense of (It opens with TextEditor but its mostly nonsensical):
qiime feature-table filter-samples --i-table ../deblur-filttable4.qza --m-metadata-file sample-metadata-dconly.tsv --o-filtered-table dconly-table
qiime tools export --input-path dconly-table.qza --output-path dconly-tableexp
Clearly I am doing something wrong and I can't seem to figure out what on my own. All help is greatly appreciated!
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### Author:  [@jphagen](https://forum.qiime2.org/u/jphagen)
#### Post date: [November 15, 2023, 9:47pm UTC](https://forum.qiime2.org/t/how-to-extract-sequences-for-specific-samples-in-dataset/28310/2 "2023-11-15T21:47:56Z")
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### Author:  [@jphagen](https://forum.qiime2.org/u/jphagen)
#### Post date: [November 15, 2023, 10:08pm UTC](https://forum.qiime2.org/t/how-to-extract-sequences-for-specific-samples-in-dataset/28310/3 "2023-11-15T22:08:27Z")
Hi @ag1170,
You are very close, thanks for putting in the work to learn Qiime2! You need to take the output of your `qiime feature-table filter-samples` and put it into `qiime feature-table filter-seqs`
You will need to run the commands from [filter-table filter-seqs](https://docs.qiime2.org/2023.9/plugins/available/feature-table/filter-seqs/)
The commands should be:
```auto
qiime feature-table filter-seqs
--i-data ../deblur-repseqs.qza
--i-table ../deblur-filttable4.qza
--o-filtered-data