I have 66 samples demultiplexed Fastq.gz files generated through Illumina MiSeq technology. I have to extract fixed number of sequences i.e. 300 sequences from each sample/read.Could you help me to do this in QIIME 2, please?
Hi there @Bhagwan! Can you please provide some more detail about what it is you are hoping to accomplish here? It sounds a bit like you are trying to rarefy your raw sequences, but usually we rarefy at the feature table step. Thanks!
Yep. I know. I have to perform ANOVA with selected sequences. Therefore, I need randomly selected 300 good quality sequences.