Hi,
I am not certain on how to pick a trimming value for my 16S rRNA amplicon sequencing data. While forward sequence looks good, I am not sure about the reverse, please see the image below. From what I have read in the forum, I feel the reverse sequence should be OK without trimming as the median quality scores are pretty high throughout. What is the best way of determining a trimming value, particularly for my data. Thanks in advance for any help.
I would highly encourage you to explore a handful of different criteria and evaluate their impact on this study, in particular taxonomic classification, differential abundance, and diversity. This will be the best way (IMO) for you to understand the impact of trim and trunc params.
Okay, so maybe it would help for us to give you some concrete starting point:
Maybe as a first pass I would use Q30 (median) as a starting point for these data. So, eyeballing from the static image you provided above: