# how to deal with technical replicates

**URL:** https://forum.qiime2.org/t/how-to-deal-with-technical-replicates/19837
**Category:** General Discussion
**Created:** [June 8, 2021, 4:43pm UTC](https://forum.qiime2.org/t/how-to-deal-with-technical-replicates/19837 "2021-06-08T16:43:16Z")
**Posts on this page:** 1
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### Author: ![timanix](https://forum.qiime2.org/user_avatar/forum.qiime2.org/timanix/32/17879_2.png) [@timanix](https://forum.qiime2.org/u/timanix)
#### Post date: [June 8, 2021, 5:39pm UTC](https://forum.qiime2.org/t/how-to-deal-with-technical-replicates/19837/2 "2021-06-08T17:39:35Z")

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Hi, Ming!  
Currently I am analyzing a rumen microbiome as well 👋.  
I am treating rumen fluid samples from different cows as biological replicates. I did not pool DNA in one sample, neither I merge them in my table. To perform any statistical analysis, or to get an overview of microbiome it is better to have replicates, rather than pool them together.

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