# How to build an OTU table using ONT sequencing

**URL:** https://forum.qiime2.org/t/how-to-build-an-otu-table-using-ont-sequencing/33624
**Category:** User Support
**Created:** [August 26, 2025, 3:40pm UTC](https://forum.qiime2.org/t/how-to-build-an-otu-table-using-ont-sequencing/33624 "2025-08-26T15:40:14Z")
**Posts on this page:** 1
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### Author: ![gregcaporaso](https://forum.qiime2.org/user_avatar/forum.qiime2.org/gregcaporaso/32/17769_2.png) [@gregcaporaso](https://forum.qiime2.org/u/gregcaporaso)
#### Post date: [August 27, 2025, 4:56pm UTC](https://forum.qiime2.org/t/how-to-build-an-otu-table-using-ont-sequencing/33624/7 "2025-08-27T16:56:56Z")

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Hi @ana_btt, Have you reviewed this post yet:

> [@PRONAME: Enhancing Taxonomic Accuracy with Nanopore Long-Read Metabarcoding and QIIME2](https://forum.qiime2.org/t/proname-enhancing-taxonomic-accuracy-with-nanopore-long-read-metabarcoding-and-qiime2/32304):
>
> Hi everyone! Here is a post for those of you interested in combining the strengths of both QIIME2 and Nanopore long-read metabarcoding raised_hands. As you may know, the sequencing technology developed by Oxford Nanopore Technologies (ONT) enables the generation of (very) long reads, which can significantly enhance the taxonomic resolution of metabarcoding approaches mag_right. For example, the entire 16S-ITS-23S operon region can be sequenced in one go. However, Nanopore sequencing has hi…

That might help you get started. Additionally there are some ONT-focused plugins on the horizon, but nothing in the distros or on the QIIME 2 Library yet.

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