Dear Sir, The Image has been attached with this reply. I am connecting laptop with a server, The main directory that I have created is Abbas and for analysis I have used paired end sequence format as mentioned in QIIME2 website. May I am unable to create input path for this data. If the main directory is Abbas what should be the input path and what should be the format for the attached files in QIIME2. I mean where to start, I just make a main directory named as Abbas later I directly put the format mentioned in your website and failed.