# How I check the quality score in reference data?

**URL:** https://forum.qiime2.org/t/how-i-check-the-quality-score-in-reference-data/5268
**Category:** User Support
**Tags:** demux
**Created:** [July 26, 2018, 5:37am UTC](https://forum.qiime2.org/t/how-i-check-the-quality-score-in-reference-data/5268 "2018-07-26T05:37:22Z")
**Posts on this page:** 5
**Page:** 1

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### Author: ![Don](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/d/ed655f/32.png) [@Don](https://forum.qiime2.org/u/Don)
#### Post date: [July 26, 2018, 5:37am UTC](https://forum.qiime2.org/t/how-i-check-the-quality-score-in-reference-data/5268/1 "2018-07-26T05:37:22Z")

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Hi, Im studying microbial eukaryotic diversity in the Korea.

I prepared reference data this way.

qiime tools import   
--type 'FeatureData[Sequence]'   
--input-path /Users/lompe/murder/pr2.fasta   
--output-path /Users/lompe/murder/pr2.qza

qiime feature-classifier extract-reads   
--i-sequences /Users/lompe/Qiimetest/pr2.qza   
--p-f-primer CCAGCASCYGCGGTAATTCC   
--p-r-primer ACTTTCGTTCTTGATYRA   
--o-reads /Users/lompe/Qiimetest/v4\_extracts.qza

The problem is how I check the quality score in v4\_extracts.qza

This is a error message.

(qiime2-2018.6) LabOfMarineProtistanEcophysioloygy:Qiimetest lompe$ qiime demux summarize \

> --i-data v4\_extracts.qza   
> --o-visualization v4\_extracts.qzv  
> Plugin error from demux:

Argument to parameter 'data' is not a subtype of SampleData[JoinedSequencesWithQuality | PairedEndSequencesWithQuality | SequencesWithQuality].

Debug info has been saved to /var/folders/kv/pkjw8\_2d2\_x9ydbtrbt\_ns9c0000gn/T/qiime2-q2cli-err-3xuob14i.log

And I tried make demux.qza by reference data. But I had a no barcode file and metadata.

Please help me, thanks.

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [July 26, 2018, 12:04pm UTC](https://forum.qiime2.org/t/how-i-check-the-quality-score-in-reference-data/5268/2 "2018-07-26T12:04:52Z")

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [July 26, 2018, 12:08pm UTC](https://forum.qiime2.org/t/how-i-check-the-quality-score-in-reference-data/5268/3 "2018-07-26T12:08:57Z")

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Hi @Don,  
Your input data is fasta format:

> [@Don](#):
>
> –input-path /Users/lompe/murder/pr2.fasta

fasta format does not contain any quality scores; hence it is imported as `FeatureData[Sequence]` type. This is not one of the required types for that command (which contain quality scores):

> [@Don](#):
>
> Argument to parameter ‘data’ is not a subtype of SampleData[JoinedSequencesWithQuality | PairedEndSequencesWithQuality | SequencesWithQuality].

I hope that helps clarify!

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<div class="post-metadata">

### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [July 26, 2018, 12:09pm UTC](https://forum.qiime2.org/t/how-i-check-the-quality-score-in-reference-data/5268/4 "2018-07-26T12:09:03Z")

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [August 26, 2018, 6:09pm UTC](https://forum.qiime2.org/t/how-i-check-the-quality-score-in-reference-data/5268/5 "2018-08-26T18:09:03Z")

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