# How can I run this step, when I get the overview of the read quality?

**URL:** https://forum.qiime2.org/t/how-can-i-run-this-step-when-i-get-the-overview-of-the-read-quality/33340
**Category:** Technical Support
**Tags:** moshpit, q2view
**Created:** [June 25, 2025, 5:21am UTC](https://forum.qiime2.org/t/how-can-i-run-this-step-when-i-get-the-overview-of-the-read-quality/33340 "2025-06-25T05:21:52Z")
**Posts on this page:** 4
**Page:** 1

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### Author: ![yangyue](https://forum.qiime2.org/user_avatar/forum.qiime2.org/yangyue/32/19097_2.png) [@yangyue](https://forum.qiime2.org/u/yangyue)
#### Post date: [June 25, 2025, 5:21am UTC](https://forum.qiime2.org/t/how-can-i-run-this-step-when-i-get-the-overview-of-the-read-quality/33340/1 "2025-06-25T05:21:52Z")

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I'm using the tutorial of QIIME2 Moshpit version 2025.4, and I'm sure I've finished the "[Data retrieval]", but I want to know how "demux summary" works, the code as following:

 ![图片](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/8/5/8584718ed83e7fbbbaea1c76e8d8fd393bb5cc14.png).  
My warnings as below:  
 ![图片](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/4/2/4240c332a532148797cb4f04814304c9d6b1f45c.png).

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### Author: ![timanix](https://forum.qiime2.org/user_avatar/forum.qiime2.org/timanix/32/17879_2.png) [@timanix](https://forum.qiime2.org/u/timanix)
#### Post date: [June 25, 2025, 6:50am UTC](https://forum.qiime2.org/t/how-can-i-run-this-step-when-i-get-the-overview-of-the-read-quality/33340/2 "2025-06-25T06:50:08Z")

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Hello!

> [@yangyue](#):
>
> I'm using the tutorial of QIIME2 Moshpit version 2025.4, and I'm sure I've finished the "[Data retrieval]",

Assuming that you are just training and using the same commands as in the tutorial, the outputs should be...

```auto

mosh fondue get-all \
    --i-accession-ids ./cache:ids \
    --p-email YOUR.EMAIL@domain.com \
    --p-threads 5 \
    --p-retries 5 \
    --o-paired-reads ./cache:reads_paired \
    --o-metadata ./cache:metadata \
    --o-single-reads ./cache:reads_single \
    --o-failed-runs ./cache:failed_runs \
    --verbose

```

... either ./cache:reads\_paired or ./cache:reads\_single

You can try ./cache:reads\_paired first and if there is nothing try ./cache:reads\_single.

If you modifyed the abovementioned command, make sure that you use the same tag for the following command you run.

Best,

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### Author: ![yangyue](https://forum.qiime2.org/user_avatar/forum.qiime2.org/yangyue/32/19097_2.png) [@yangyue](https://forum.qiime2.org/u/yangyue)
#### Post date: [June 27, 2025, 2:05pm UTC](https://forum.qiime2.org/t/how-can-i-run-this-step-when-i-get-the-overview-of-the-read-quality/33340/3 "2025-06-27T14:05:40Z")

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Thank you!  
It is a common point I always neglect. I think it may a common phenonmenon every Qiime2 tutorial user likes to paste and use the code without thinking when they first training their new Qiime2 skills.

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [July 28, 2025, 8:05pm UTC](https://forum.qiime2.org/t/how-can-i-run-this-step-when-i-get-the-overview-of-the-read-quality/33340/4 "2025-07-28T20:05:51Z")

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