# How can I process MD5sum format sequence data in the QIIME2? **URL:** https://forum.qiime2.org/t/how-can-i-process-md5sum-format-sequence-data-in-the-qiime2/19100 **Category:** User Support **Created:** [April 5, 2021, 3:34pm UTC](https://forum.qiime2.org/t/how-can-i-process-md5sum-format-sequence-data-in-the-qiime2/19100 "2021-04-05T15:34:44Z") **Posts on this page:** 1 **Showing post:** 4
### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer) #### Post date: [April 15, 2021, 8:29pm UTC](https://forum.qiime2.org/t/how-can-i-process-md5sum-format-sequence-data-in-the-qiime2/19100/4 "2021-04-15T20:29:54Z")
> [@laxmi\_sharma](#): > > I tried to create a manifest file as instructed in [Importing data — QIIME 2 2021.2.0 documentation](https://docs.qiime2.org/2021.2/tutorials/importing/#manifest-file). But it seems I am doing some mistakes. Can you let us know what issues / errors you are running into? Often users have trouble getting :qiime2: (running within WSL) to find their own files that exist in a Windows path, which is not easily understood by WSL. I'd recommend reading through the following posts: > [@Import data problem](https://forum.qiime2.org/t/import-data-problem/18451/3): > > Hi @Audrey_Anne, I think you are trying to use Windows command prompt syntax in your Ubuntu LTS. That is, this: Users\audre\Bureau\Projetmain should be: Users/audre/Bureau/Projetmain One trick that you can use to share files between your Windows and Ubuntu LTS is to do the following: In Ubuntu LTS terminal window type: explorer.exe . . This will open a Windows Explorer window with the path to your home directory within your Ubuntu LTS. See [here](https://forum.qiime2.org/t/qiime2-on-windows-subsystem-for-linux/12806). Drag this path in the window to your Quick… > [@Permission denied when importing FASTQ files](https://forum.qiime2.org/t/permission-denied-when-importing-fastq-files/18490/13): > > Hi @taf1g17, This is very helpful thank you! As I mentioned earlier is this thread, you can not use Windows-style file paths, but the file paths as WSL understands them. The best way to make sure you are using the correct Linux file paths is to cd into the folder where your sequence files are located, and type pwd. This will provide you with the absolute directory path. Do this within the WSL Terminal of course. wink taf1g17$: cd share/seqs-to-import taf1g17:~/share/seqs-to-import$ pwd … > [@Trying to install QIIME 2 2020.11 on Windows](https://forum.qiime2.org/t/trying-to-install-qiime-2-2020-11-on-windows/17812/6): > > So when I see the C:\ as you have here: That tells me that you are running through the Windows command prompt and not WSL (i.e. there should be a separate "app" icon for you to launch WSL. In my case I have an icon for Ubuntu 18.04 LTS. The thread I linked you above describes this (note the screen-shot of the icons). To clarify, on my Windows 10 setup: my Windows 10 Command Prompt looks like: C:\Users\Mike\> my WSL prompt looks like: (base) mike@BLUE:~$ That is, it appears that you ha… -Mike --- _[View the full topic](https://forum.qiime2.org/t/how-can-i-process-md5sum-format-sequence-data-in-the-qiime2/19100)._