Hello!
I have one fastq with 29 samples, only 6 of them are mine. It was used Ion torrent to sequencing. I used the fastq manifest to import the data to qiime 2 (Im using the 2020.2 version). And this .qza file have the barcodes and primers in it.
How do I take them out?
I have tied the cutadapt demux-single, but appears the error:
Invalid value for “–i-seqs”: Expected an artifact of at least type
MultiplexedSingleEndBarcodeInSequence. An artifact of type
SampleData[SequencesWithQuality] was provided.
What I actually have is one file fastq with 29 samples. So yes I will try to import them with the cutadapt plugin that you mention! The file name is R_2019_11_13_19_51_37_user_SN2-93-Etchebehere_Vaz_AlejandraLatu_16S_Arquea_13_11_2019.fastq, and contains all the samples.
I understood that was the one that it worked for my type of data to import it.
Thanks again! Hope it work!!