I would apprecciate some help with an error I've gotten while trying to import some paired-end files from a MiSeq running.
I'm using qiime2-2021.4, which I installed using conda.
I have used the following command:
qiime tools import --type 'SampleData[PairedEndSequencesWithQuality]' --input-path Unmerged/manifest_PE_file.tsv --output-path 16S-paired-end.qza --input-format PairedEndFastqManifestPhred33V2
Then I got the following error:
There was a problem importing Unmerged/manifest_PE_file.tsv:
Unmerged/manifest_PE_file.tsv is not a(n) PairedEndFastqManifestPhred33V2 file:
Filepath on line 1 and column "forward-absolute-filepath" could not be found (~/Documents/MiSeq_16S/Unmerged/ANT001003_S58_L001_R1_001.fastq.gz) for sample "ANT001003".
My manifest file is available here: Manifest - Google Sheets
Thank you in advance!