i use the example data result from the error occurred when i run this code with my own data, and last month when i run the same code in another computer there are no error, so i am wondering if my qiime2 is wrong or some other problem?
It looks like this is a single end manifest file, rather than a paired end file (which is what the rror is telling you.) So, its possible you might have downloaded the wrong file. Try looking at the paired end manifest and seeing if that works better for you.