I am requesting so assistance with importing fastq files I recently received. There are only two files that contain the Forward and Reverse reads of all of my samples. I believe that the sequences have already been demultiplexed. Also these sequences have the barcode included in the header, and I do not have a separate barcodes.fastq file.
I am struggling with how to import these data as none of the tutorials on the Import help page seem to apply.
Here is some of the code I have run:
qiime tools import --type EMPPairedEndSequences --input-path 16S --output-path emp-paired-end-sequences.qza
There was a problem importing 16S:
Missing one or more files for EMPPairedEndDirFmt: 'barcodes.fastq.gz’
qiime tools import --type ‘SampleData[PairedEndSequencesWithQuality]’ --input-path 16s --output-path demux-paired-end.qza
There was a problem importing 16s:
Missing one or more files for SingleLanePerSamplePairedEndFastqDirFmt: '.+_.+_L[0-9][0-9][0-9]_R_001\.fastq\.gz
Thank you for your help!