I am running qiime 2 from the command line (q2cli version 2017.12.0).
I am using the chronic fatigue syndrome tutorial (https://docs.qiime2.org/2017.12/tutorials/gneiss/) and when I run the following line:
qiime gneiss add-pseudocount --i-table table.qza --p-pseudocount 1 --o-composition-table composition.qza
I get this error:
Usage: qiime [OPTIONS] COMMAND [ARGS]…
Error: No such command “gneiss”.
Prior to running this line, I activated qiime2
source activate qiime2-2017.12
I am also working within a virtual environment.
When I run qiime --info I get:
Python version: 3.5.4
QIIME 2 release: 2017.12
QIIME 2 version: 2017.12.1
q2cli version: 2017.12.0
No plugins are currently installed.
You can browse the official QIIME 2 plugins at https://qiime2.org
Application config directory
To get help with QIIME 2, visit https://qiime2.org
Citing QIIME 2
If you use QIIME 2 in any published work, you should cite QIIME 2 and the plugins that you used. To display the citations for QIIME 2 and all installed plugins, run:
qiime info --citations
Any help figuring out why I can’t run gneiss would be much appreciated!
Hi @Calliope_Holingue! How did you install QIIME 2? For example, did you perform a native installation, or are you using one of the virtual machine options (e.g. VirtualBox, Docker, AWS)?
What kind of virtual environment are you using (e.g. Python
virtualenv, conda environment, something else)? Depending on what you have set up, the virtual environment could be causing issues with the QIIME 2 installation.
Hi @jairideout, thanks for your help.
I installed QIIME 2 using native installation, as described in the link you provided. I am running QIIME 2 within a conda environment, again based on the link above (source activate qiime2-2017.12),
Thanks for the info! I’m not sure why none of the QIIME 2 plugins are installed, something must have gone awry during the install process. Can you please try removing your
qiime2-2017.12 environment and reinstall QIIME 2 following the native installation guide? After removing the
qiime2-2017.12 environment, and before installing the environment again, please run these commands to clear conda’s cache and update conda:
conda clean -y --all
conda update conda
If reinstalling QIIME 2 doesn’t solve the problem, can you please send me the exact install commands you’re running, and all of the output they produce? Also let me know what operating system you’re using (macOS or Linux). Thanks!
Hi @jairideout, thanks for the help! The issue was resolved. I’m working from a cluster and had a to create a user specific conda environment to run qiime2, because the cluster version didn’t allow for all the qiime2 dependencies.
It’s working great now. Thanks!
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