# galaxy qiime 2 tools import fastq data **URL:** \ **Category:** Technical Support\ **Created:** [October 5, 2022, 12:07pm UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355 "2022-10-05T12:07:17Z")\ **Posts on this page:** 9\ **Page:** 1
**Author:** ![sdavid](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/s/9fc29f/32.png) [@sdavid](https://forum.qiime2.org/u/sdavid)\ **Post date:** [October 5, 2022, 12:07pm UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355/1 "2022-10-05T12:07:17Z")
Hi I try to use qiime2 in galaxy but I have a problem with importing data I 'm trying to reproduce my script on galaxy but I don't understand what input I need to import my reads. I tried with my manifest using with my terminal. but it didn't work I have an error on galaxy windows on the right of my screen (below) Unexpected error importing data: /export/galaxy-central/database/files /000/dataset\_12.dat is not a(n) PairedEndFastqManifestPhred33 file: File referenced on line 2 could not be found (/Users/slegall/Desktop/R1R2 \_souris\_Salmo\_Avril\_2022//SD-21S1\_TCG TTC and when I click on this error Unexpected error importing data: /export/galaxy-central/database/files /000/dataset\_12.dat is not a(n) PairedEndFastqManifestPhred33 file: File referenced on line 2 could not be found (/Users/slegall/Desktop/R1R2 \_souris\_Salmo\_Avril\_2022//SD-21S1\_TCG TTC-K76YR\_L001\_R1.fastq.gz). ☹ ｢type: SampleData[PairedEndSequencesWithQuality]｣ ｢format: PairedEndFastqManifestPhred33｣ I tried to change path in my document, changed csv in tsv ... Can You explain to me what input I need to make the first qza : "paired-end-demux.qza" I have pairedendfastq reads Thanks a lot Regards Sandrine ---
**Author:** ![Oddant1](https://forum.qiime2.org/user_avatar/forum.qiime2.org/oddant1/32/17130_2.png) [@Oddant1](https://forum.qiime2.org/u/Oddant1)\ **Post date:** [October 5, 2022, 10:25pm UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355/2 "2022-10-05T22:25:58Z")
---
**Author:** ![Oddant1](https://forum.qiime2.org/user_avatar/forum.qiime2.org/oddant1/32/17130_2.png) [@Oddant1](https://forum.qiime2.org/u/Oddant1)\ **Post date:** [October 5, 2022, 10:29pm UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355/3 "2022-10-05T22:29:12Z")
Hello @sdavid, you will need to upload all of your data in the `R1R2 _souris_Salmo_Avril_2022` directory to galaxy as a collection using the `Upload Data` tool. Then you will need to use `qiime tools import` to import it as `SampleData[PairedEndSequencesWithQuality]` in the `Casava One Eight Single Lane Per Sample Directory Format` format. Instructions on how to do this may be found [here](https://docs.qiime2.org/jupyterbooks/cancer-microbiome-intervention-tutorial/020-tutorial-upstream/030-importing.html#importing). ---
**Author:** ![lizgehret](https://forum.qiime2.org/user_avatar/forum.qiime2.org/lizgehret/32/17128_2.png) [@lizgehret](https://forum.qiime2.org/u/lizgehret)\ **Post date:** [October 5, 2022, 10:33pm UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355/4 "2022-10-05T22:33:45Z")
---
**Author:** ![sdavid](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/s/9fc29f/32.png) [@sdavid](https://forum.qiime2.org/u/sdavid)\ **Post date:** [October 17, 2022, 8:01am UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355/5 "2022-10-17T08:01:59Z")
Hi It doesn’t work I have this error message I send you my table Is there another possibility to upload paired end sequences in galaxy qiime2 in tar format for exemple like in Frogs Thanks Regards Sandrine ![GraphiqueCollé-1](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/9/9092a61ff34373ef0a0a57f9fa4a5989e11553f8.jpeg) ![Capture d’écran 2022-10-17 à 09.56.53](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/5/5f5ad0a88f8a4ed0e65d2f1475aeabe45246f50e.png) ---
**Author:** ![lizgehret](https://forum.qiime2.org/user_avatar/forum.qiime2.org/lizgehret/32/17128_2.png) [@lizgehret](https://forum.qiime2.org/u/lizgehret)\ **Post date:** [October 18, 2022, 3:24pm UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355/6 "2022-10-18T15:24:57Z")
---
**Author:** ![ebolyen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ebolyen/32/11_2.png) [@ebolyen](https://forum.qiime2.org/u/ebolyen)\ **Post date:** [October 19, 2022, 5:33pm UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355/7 "2022-10-19T17:33:42Z")
---
**Author:** ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)\ **Post date:** [November 19, 2022, 11:34pm UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355/8 "2022-11-19T23:34:35Z")
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed. ---
**Author:** ![ebolyen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ebolyen/32/11_2.png) [@ebolyen](https://forum.qiime2.org/u/ebolyen)\ **Post date:** [October 1, 2024, 6:55pm UTC](https://forum.qiime2.org/t/galaxy-qiime-2-tools-import-fastq-data/24355/9 "2024-10-01T18:55:25Z")