# Fungal ITS analysis tutorial

**URL:** https://forum.qiime2.org/t/fungal-its-analysis-tutorial/7351
**Category:** Tutorials
**Created:** [December 9, 2018, 2:56pm UTC](https://forum.qiime2.org/t/fungal-its-analysis-tutorial/7351 "2018-12-09T14:56:30Z")
**Posts on this page:** 1
**Showing post:** 15

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### Author: ![gkphilip](https://forum.qiime2.org/user_avatar/forum.qiime2.org/gkphilip/32/11085_2.png) [@gkphilip](https://forum.qiime2.org/u/gkphilip)
#### Post date: [February 1, 2022, 12:31am UTC](https://forum.qiime2.org/t/fungal-its-analysis-tutorial/7351/15 "2022-02-01T00:31:50Z")

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Thanks for your really helpful tutorial. One issue I had running it with my own data was doing cutadapt in one step as it didn't trim the adapters correctly. Running it in two steps seems to work though. I think there have been a few other reports of this on the forum.

In case you're interested in the original issue and solutions: [ITS cutadapt trimming of primer and reverse complement of the reverse primer](https://forum.qiime2.org/t/its-cutadapt-trimming-of-primer-and-reverse-complement-of-the-reverse-primer/21344/) and [cutadapt / trim-paired / option "front" and "adapter"](https://forum.qiime2.org/t/cutadapt-trim-paired-option-front-and-adapter/21372) and [Cut-adapt trim paired - different results when primers separate vs linked](https://forum.qiime2.org/t/cut-adapt-trim-paired-different-results-when-primers-separate-vs-linked/18734).

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