From DADA2 to dereplication; error QIIME1DemuxFormat filea[Sequences]

[cherman2 (Chloe Herman)]

Hey @srosales712
Thanks for the additional information! :qiime2:
So I personally don't have a lot experience merging 2 different primers but I looked into posts on the forum and I think I found some interesting discussions that might be better then dereplicating again.

This post seems like it is doing something very similar to what you are doing

Combining data from different sequencing centers and primers

If we used two sequencing centers that used different primers (V4 only and V3+V4), would it be fine to generate two separate DADA2 feature tables, then merge the feature tables later to analyze the samples together? All samples are unique in both data sets.

This one is a little older but has some interesting discussion on it( although I don't think as relevant )

Combining Datasets with 2 sets of Primers

I have two sets of data (paired reads), with two different primer sets (341/806 and 515/806) that I need to combine. I would like to try trimming my 341/806 set to 515/806 but I’m not sure how to go about doing so before pipelining into dada2. Thank you! Julie

Both mention and link to Fragment insertion. Here is another link .
https://library.qiime2.org/plugins/q2-fragment-insertion/16/

Let me know if this is helpful or if I am way off the mark.
Chloe