# Follow up to - Demux summarize error: Plugin error from demux- 'NoneType' object has no attribute 'strip' **URL:** \ **Category:** User Support\ **Tags:** dada2, summarize\ **Created:** [July 2, 2018, 8:19pm UTC](https://forum.qiime2.org/t/follow-up-to-demux-summarize-error-plugin-error-from-demux-nonetype-object-has-no-attribute-strip/4844 "2018-07-02T20:19:58Z")\ **Posts on this page:** 6\ **Page:** 1
**Author:** ![venkar](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/v/8e7dd6/32.png) [@venkar](https://forum.qiime2.org/u/venkar)\ **Post date:** [July 2, 2018, 8:19pm UTC](https://forum.qiime2.org/t/follow-up-to-demux-summarize-error-plugin-error-from-demux-nonetype-object-has-no-attribute-strip/4844/1 "2018-07-02T20:19:58Z")
Hi, I have the same problem as already describes in this thread - [Demux summarize error: Plugin error from demux- 'NoneType' object has no attribute 'strip' - #10 by taknotts](https://forum.qiime2.org/t/demux-summarize-error-plugin-error-from-demux-nonetype-object-has-no-attribute-strip/1244/10). I checked to see that I did have one too many or too few sequences after looking at the divisibility of total number of sequences by 4. I have 300 fastq.gz files and I may not be able to investigate one by one. Is there a solution to summarize my demux.qza file ? Also, I am using dada2 to perform sequence variants. So far it is running okay. Should I expect any errors ? Thanks, Raghavee ---
**Author:** ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)\ **Post date:** [July 3, 2018, 2:06pm UTC](https://forum.qiime2.org/t/follow-up-to-demux-summarize-error-plugin-error-from-demux-nonetype-object-has-no-attribute-strip/4844/2 "2018-07-03T14:06:04Z")
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**Author:** ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)\ **Post date:** [July 4, 2018, 12:25am UTC](https://forum.qiime2.org/t/follow-up-to-demux-summarize-error-plugin-error-from-demux-nonetype-object-has-no-attribute-strip/4844/3 "2018-07-04T00:25:52Z")
Hey there @venkar! > [@venkar](#): > > I have 300 fastq.gz files and I may not be able to investigate one by one Fortunately QIIME 2 can do this for you! ```bash qiime tools validate demux.qza ``` If you can run that command on the Artifact in question and provide the results here, that will be a great start! Thanks! :qiime2: 🦖 ---
**Author:** ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)\ **Post date:** [July 4, 2018, 12:25am UTC](https://forum.qiime2.org/t/follow-up-to-demux-summarize-error-plugin-error-from-demux-nonetype-object-has-no-attribute-strip/4844/4 "2018-07-04T00:25:58Z")
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**Author:** ![venkar](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/v/8e7dd6/32.png) [@venkar](https://forum.qiime2.org/u/venkar)\ **Post date:** [July 10, 2018, 4:48pm UTC](https://forum.qiime2.org/t/follow-up-to-demux-summarize-error-plugin-error-from-demux-nonetype-object-has-no-attribute-strip/4844/5 "2018-07-10T16:48:23Z")
Perfect. I had only one sample that did not have the right number of lines, Hence I discarded that. I ran dada2 and it completed without any error after removing the offending sample. Thanks! ---
**Author:** ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)\ **Post date:** [August 10, 2018, 10:48pm UTC](https://forum.qiime2.org/t/follow-up-to-demux-summarize-error-plugin-error-from-demux-nonetype-object-has-no-attribute-strip/4844/6 "2018-08-10T22:48:24Z")
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