File does not exist. Why?

[Mehrdad (Meha)]

I double-checked my parameters and also I copied and pasted the file's names and so on.
Why it say the file is not present?

Thanks a lot

[thermokarst]

Hi @Mehrdad!

@Mehrdad

Why it say the file is not present?

Simple - because you don't have a file named importedlibA in your folder --- you have a file called importedlibA.qza --- you forgot to include the .qza from the name. The same problem is present for your metadata file. The command should actually be this, based on the names you have provided above:

qiime cutadapt demux-single \
  --i-seqs importedlibA.qza \
  --m-barcodes-file Metadataforw1and4.tsv \
  --m-barcodes-column staggerandbarcode \
  --o-per-sample-sequences demux.qza \
  --o-untrimmed-sequences untrimmed.qza \
  --verbose

Also note I added --o-per-sample-sequences --- you forgot to include that in your command above --- so, once you fixed your filenames, that would've been an issue, too.

[Mehrdad (Meha)]

Dear Matthew you rock!
Awesome!
Thank you very much!