Hello
I am trying to train my classifier for taxonomy.
I used this command below to denoising my data
qiime dada2 denoise-paired
–i-demultiplexed-seqs collabration-demux-paired-end.qza
–p-trim-left-f 6
–p-trim-left-r 5
–p-trunc-len-f 300
–p-trunc-len-r 223
–p-n-threads 4
–o-table collabration-table.qza
–o-representative-sequences collabration-rep-seqs.qza
–o-denoising-stats collabration-denoising-stats.qza &
and this is my primers information
Target
341F - 806R
Forward Primer(341F)
CCTAYGGGRBGCASCAG
ReversePrimer(806R)
GGACTACNNGGGTATCTAAT
ReadLength
300bpPE
My question is when I am trying to run the feature classifier part using the command belove, what should I put for the p-trunc-len part? The way I do, is that correct?
qiime feature-classifier extract-reads
–i-sequences 99_otus.qza
–p-f-primer CCTAYGGGRBGCASCAG
–p-r-primer GGACTACNNGGGTATCTAAT
–p-trunc-len-f 300 *
** --p-trunc-len-r 223 *
–o-reads collaborator-ref-seqs.qza