fastq file import

Hi, I am using trying to import my minifest file into the qiime2 analysis, it always give a feedback as “manifest_COI_m5.txt is not a(n) PairedEndFastqManifestPhred33V2 file”.

I have tried my best to make my manifest file formatted according to the requirement of qiime2.

Here is my script:
qiime tools import --type ‘SampleData[PairedEndSequencesWithQuality]’ --input-path manifest_COI_m5.txt --output-path paired-end-demux.qza --input-format PairedEndFastqManifestPhred33V2
manifest_COI_m5.txt (21.6 KB)
Attached is my manifest file, could you please let me know a better way to generate the manifest file?
Thanks!

Hi @yuanyuan12543,

Welcome to the :qiime2: forum!

You manifest format is an earlier version. Try importing your data as PairedEndFastqManifestPhred33, not PairedEndFastqManifestPhred33V2.

Best,
Justine

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Oh, my world! It works. You saved me.
Thank you very much!

Junli

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