Hi, I would like to import reference-hit.seqs.fa to QIIME2. I can import reference-hit.biom successful. but when I use qiime tools import --input-path reference-hit.seqs.fa --output-path rep-seqs.qza --type FeatureData[Sequence]. It reported the reference-hit.seq.fa is not a Featuredata[Sequence] …

Hi @Jingsi_Tang ! Can you please provide the following info? How did you generate the two files you're trying to import? i.e. reference-hit.seqs.fa and reference-hit.biom Can you supply the first few lines of reference-hit.seqs.fa? Can you run qiime feature-table summarize on the .qza file y…

Hi @jairideout Thank you for your response!! 1.How did you generate the two files you’re trying to import? i.e. reference-hit.seqs.fa and reference-hit.biom my data are demultiplexed merged pair end data, and each sample have one fasta.file or fastq.file. deblur workflow --seqs-fp 01.CleanData -…

Hi, @jairideout This is my .biom.qzv file. table.qzv (680.4 KB) Thanks.

Hi @Jingsi_Tang , I have a couple of theories of what could be going on, could you send us the result of the commands below? Just copy/paste the lines below in the terminal, while you are in the same folder than your reference-hit.seqs.fa file. wc -l reference-hit.seqs.fa grep -v '^>' reference-hit…

Hi, @antgonza Thank you for your response. This is the result : fdeng@JZHAO-G01DU:~/projects/2017_11_21_tang/clean_data$ wc -l reference-hit.seqs.fa 7848 reference-hit.seqs.fa fdeng@JZHAO-G01DU:~/projects/2017_11_21_tang/clean_data$ grep -v '^>' reference-hit.seqs.fa | wc -l 3924 fdeng@JZHAO-…

OK, out of ideas. Could you share the file with us? BTW, which version of qiime2 are you using?

Hi, @antgonza This is the qiime 2017.10.0 version Thanks

At this point, the only way to figure the issue out is by taking a close look to your reference-hit.seqs.fa, could you share it? Also, to be sure, if you could send us the md5 checksum of that file, that will be great!

Hi @antgonza How to make the md5 checksum of my reference-hit.seqs.fa ? I am sorry I am not good at it... Thanks...

Hi @Jingsi_Tang ! I want to take a quick step back --- it looks like you used deblur, instead of q2-deblur to generate the files you are attempting to import. It looks like q2-deblur does a little bit of cleanup and manipulation of the files it produces in order for them to be saved as QIIME 2 Artifa…

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Fasta.files from deblur failed to import QIIME2 about generate rep-seqs.qza

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