Hi there,
my metadata constains samples from 4 differents studies, I have run the same code for the other 3 studies with the same metadata file and they worked, I am not sure why it is not working only for the 5 samples from this study.
below I have attached an image from my metadata, and also the file itself. sample-metadata.tsv (18.5 KB)
@lilycrook, this error often occurs when there are no groups for alpha-group-significance to compare between. It looks like this particular study is happening on a subset of the samples in the metadata?
Any chance that all of the categorical metadata columns are the same for those 5 samples?