# Faith\_pd visualization in Alpha diversity

**URL:** https://forum.qiime2.org/t/faith-pd-visualization-in-alpha-diversity/8033
**Category:** User Support
**Tags:** metadata, alpha
**Created:** [January 28, 2019, 8:59am UTC](https://forum.qiime2.org/t/faith-pd-visualization-in-alpha-diversity/8033 "2019-01-28T08:59:56Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![kyeong\_yun](https://forum.qiime2.org/user_avatar/forum.qiime2.org/kyeong_yun/32/4365_2.png) [@kyeong\_yun](https://forum.qiime2.org/u/kyeong_yun)
#### Post date: [January 28, 2019, 8:59am UTC](https://forum.qiime2.org/t/faith-pd-visualization-in-alpha-diversity/8033/1 "2019-01-28T08:59:57Z")

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Hi,  
I having some problem to generate alpha diversity.  
Until this step everything is ok, but when I do this command:  
qiime diversity alpha-group-significance  
--i-alpha-diversity fiath\_pd\_vector.qza  
--m-metadata-file metadatafile.tsv  
--o-visualization faith\_pd\_vector.qzv

I receive error message:

Plugin error from diversity:

Metadata does not contain any columns that satisfy this visualizer's requirements. Theremust be at least one metadata column that contains categorical data, isn't empty, doesn't consist of unique values, and doesn't consist of exactly one value.

Debug info has been saved to /tmp/qiime2-q2cli-err-5hmp4\_c9.log

I have two samples and metadata.tsv is as follows.

 ![metadata](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/b/b03ad0a1a15560b66150b207a7fc7bba5801ee11.png)

I did the validation on 'keemei' in google sheets.

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### Author: ![jwdebelius](https://forum.qiime2.org/user_avatar/forum.qiime2.org/jwdebelius/32/9655_2.png) [@jwdebelius](https://forum.qiime2.org/u/jwdebelius)
#### Post date: [January 28, 2019, 9:04am UTC](https://forum.qiime2.org/t/faith-pd-visualization-in-alpha-diversity/8033/2 "2019-01-28T09:04:35Z")

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Hi @kyeong_yun ,

It sounds like you don't have enough data for a comparison. Your columns are either all unique values (`#SampleID`, `Description`, and `Type`) or only consist of one value (`BodySite`).

You're going to have a really hard time doing any statistics with just two samples. (We had a big discussion about some of that [here](https://forum.qiime2.org/t/comparing-beta-diversity-between-samples-not-groups/7981), recently.) However, the long and short of it is that you can't do statitics with just two samples, and that there are no comparisons to be made.

Best,  
Justine

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [February 28, 2019, 3:04pm UTC](https://forum.qiime2.org/t/faith-pd-visualization-in-alpha-diversity/8033/3 "2019-02-28T15:04:40Z")

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