# Extracting V4 region from V3-V4 data

**URL:** https://forum.qiime2.org/t/extracting-v4-region-from-v3-v4-data/13575
**Category:** User Support
**Created:** [February 12, 2020, 12:42am UTC](https://forum.qiime2.org/t/extracting-v4-region-from-v3-v4-data/13575 "2020-02-12T00:42:15Z")
**Posts on this page:** 1
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### Author: ![Mehrbod\_Estaki](https://forum.qiime2.org/user_avatar/forum.qiime2.org/mehrbod_estaki/32/4001_2.png) [@Mehrbod\_Estaki](https://forum.qiime2.org/u/Mehrbod_Estaki)
#### Post date: [February 12, 2020, 1:48am UTC](https://forum.qiime2.org/t/extracting-v4-region-from-v3-v4-data/13575/2 "2020-02-12T01:48:21Z")

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Hi @ashutosh,  
Yes, you will certainly be able to accomplish this using [cutadapt trim-paired](https://docs.qiime2.org/2019.10/plugins/available/cutadapt/trim-paired/), -\> [tutorial here](https://forum.qiime2.org/t/demultiplexing-and-trimming-adapters-from-reads-with-q2-cutadapt/2313). I would recommend running the 2 datasets separately (in fact for downstream use with DADA2 this is required) and use cutadapt to remove the 515F/806R primers from both runs. I also like to add on the `--p-discard-untrimmed` parameter to get rid of any reads that didn't have these primers in them, but that's a personal choice.  
Your remaining reads should all be to that specific region.  
I'll also point you to this [previous discussion](https://forum.qiime2.org/t/reads-processing-with-different-primers/2777) on analyzing data from different regions which may of interest.

Good luck!

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