qiime2 2020.06 in virtualbox
I wanna build a v3v4 classifier, but get no result, no error. And then I add the command (–verbose), get “Alignment score and position are not consensus.”
here is my command, silva-138-99-seqs.qza downloaded from dataresource in qiime2 website.
time qiime feature-classifier extract-reads --i-sequences silva-138-99-seqs.qza --p-f-primer CCTACGGGNGGCWGCAG --p-r-primer GACTACHVGGGTATCTAATCC --o-reads ref-seqs.qza --verbose
Alignment score and position are not consensus.
real 0m22.066s
user 0m14.172s
sys 0m5.957s