# Error while summarizing command

**URL:** https://forum.qiime2.org/t/error-while-summarizing-command/11367
**Category:** User Support
**Created:** [September 3, 2019, 7:55am UTC](https://forum.qiime2.org/t/error-while-summarizing-command/11367 "2019-09-03T07:55:51Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![shashankgpt](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/s/f17d59/32.png) [@shashankgpt](https://forum.qiime2.org/u/shashankgpt)
#### Post date: [September 3, 2019, 7:55am UTC](https://forum.qiime2.org/t/error-while-summarizing-command/11367/1 "2019-09-03T07:55:51Z")

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I got an error while running the command in QIIME2-2018.11 version installed using conda.

\*\*  
qiime itsxpress trim-pair-output-unmerged --i-per-sample-sequences Run20190502\_demux-paired-end.qza --p-region ALL --p-taxa F --p-cluster-id 1.0 --p-threads 20 --o-trimmed trimmed\_exact.qza

Saved SampleData[PairedEndSequencesWithQuality] to: trimmed\_exact.qza

qiime demux summarize --i-data trimmed\_exact.qza --o-visualization trimmed\_exact.qzv\*\*

**Plugin error from demux:**

\*\* Cannot describe a DataFrame without columns\*\*

**Debug info has been saved to /tmp/qiime2-q2cli-err-gql78ig3.log**  
**(qiime2-2018.11) jsb562@pallas:/mibi/users/jsb562/ITS\_pilot/ITSxpress$**  
**(qiime2-2018.11) jsb562@pallas:/mibi/users/jsb562/ITS\_pilot/ITSxpress$ cat /tmp/qiime2-q2cli-err-gql78ig3.log**  
**Traceback (most recent call last):**  
\*\* File "/mibi/users/jsb562/miniconda2/envs/qiime2-2018.11/lib/python3.5/site-packages/q2cli/commands.py", line 274, in **call** \*\*  
\*\* results = action(**arguments)**  
\*\* File "", line 2, in summarize\*\*  
\*\* File "/mibi/users/jsb562/miniconda2/envs/qiime2-2018.11/lib/python3.5/site-packages/qiime2/sdk/action.py", line 231, in bound\_callable\*\*  
\*\* output\_types, provenance)\*\*  
\*\* File "/mibi/users/jsb562/miniconda2/envs/qiime2-2018.11/lib/python3.5/site-packages/qiime2/sdk/action.py", line 424, in _callable\_executor_\*\*  
\*\* ret\_val = self.\_callable(output\_dir=temp\_dir, **view\_args)**  
\*\* File "/mibi/users/jsb562/miniconda2/envs/qiime2-2018.11/lib/python3.5/site-packages/q2\_demux/\_summarize/\_visualizer.py", line 165, in summarize\*\*  
\*\* forward\_stats = \_compute\_stats\_of\_df(forward\_scores)\*\*  
\*\* File "/mibi/users/jsb562/miniconda2/envs/qiime2-2018.11/lib/python3.5/site-packages/q2\_demux/\_summarize/\_visualizer.py", line 92, in \_compute\_stats\_of\_df\*\*  
\*\* percentiles=[0.02, 0.09, 0.25, 0.5, 0.75, 0.91, 0.98])\*\*  
\*\* File "/mibi/users/jsb562/miniconda2/envs/qiime2-2018.11/lib/python3.5/site-packages/pandas/core/generic.py", line 6751, in describe\*\*  
\*\* raise ValueError("Cannot describe a DataFrame without columns")\*\*  
**ValueError: Cannot describe a DataFrame without columns**

I am trying to run [this](https://forum.qiime2.org/t/q2-itsxpress-a-tutorial-on-a-qiime-2-plugin-to-trim-its-sequences/5780) tutorial on my own data. Any suggestions?

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<div class="post-metadata">

### Author: ![Adam\_Rivers](https://forum.qiime2.org/user_avatar/forum.qiime2.org/adam_rivers/32/10143_2.png) [@Adam\_Rivers](https://forum.qiime2.org/u/Adam_Rivers)
#### Post date: [September 3, 2019, 12:28pm UTC](https://forum.qiime2.org/t/error-while-summarizing-command/11367/2 "2019-09-03T12:28:28Z")

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I think some of your output fasta files are missing any reads. You can useqiime tools export to inspect the output.

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [October 4, 2019, 6:28pm UTC](https://forum.qiime2.org/t/error-while-summarizing-command/11367/3 "2019-10-04T18:28:29Z")

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