error when training classifier

Dear QIIME2 User Support team:

I downloaded the greengene database gg_13_8_otus.tar.gz and got error message when running the following step saying

"Plugin error from feature-classifier:

No matches found"

qiime feature-classifier extract-reads \

–i-sequences 99_otus.qza
–p-f-primer GTGCCAGCMGCCGCGGTAA
–p-r-primer GGACTACHVGGGTWTCTAAT
–p-trunc-len 170
–p-min-length 100
–p-max-length 400
–o-reads ref-seqs.qza

Could you please help me to find out how to solve this error message?

Thanks,

Jia

Hi @Jia,
The error seems to indicate there were no matches based on those primers on the ref database you provided. Which greengenes file (99_otus.qza) exactly are you using? By chance you used the aligned rep_set one by mistake?

Hi Mehrbod,

You are right. I used the 99_otus.qza generated from the fasta file from the aligned rep_set folder by mistake. I tried the other rep_set folder and it’s working now.

Thanks,

Jia

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