Hi Nicholas,
thank you very much for the rapid reply!
I am a bit confused now. In the link you provided they recommend quality filtering before starting dada 2. Yet, I can find other posts, where quality filtering prior to DADA2 is not recommended:
DADA2 and quality filtering?
In another post they provide a linke to the DADA2 homepage where they suggest a stepwise approach for big datasets. A DADA2 workflow for Big Data (1.4 or later)
How can I do this in QIIME.
thank you so much for your help!!
kind regards,
Isabelle