error when building a phylogenetic tree, help


I just run into a similar error. I'm using Qiime2-2020.8 and I have 128GB (and 500GB for hard drive) and 16 CPUs. I'm working with 2 fasta qc files forward and reverse (each file is 25MB on average). All run well including taxonomy classification. The error showed when I run the fragment-insertion sepp. I'm including below the commands I use, the log file error I obtained and the message too.

qiime fragment-insertion sepp
--i-representative-sequences boiling_dada2_rep-seqs.qza
--i-reference-database sepp-refs-silva-128.qza
--o-tree boiling_dada2_tree.qza
--o-placements boiling_tree_dada2_placements.qza
--p-threads 14

Screen Shot 2022-02-07 at 9.03.53 PM
Screen Shot 2022-02-07 at 9.03.53 PM
1920×352 75.3 KB

Removing /tmp/tmp.MXIwpOlLjy/sepp-tmp-imXUDDyhlE
Traceback (most recent call last):
File "/opt/conda/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/", line 329, in call
results = action(**arguments)
File "", line 2, in sepp
File "/opt/conda/envs/qiime2-2020.8/lib/python3.6/site-packages/qiime2/sdk/", line 245, in bound_callable
output_types, provenance)
File "/opt/conda/envs/qiime2-2020.8/lib/python3.6/site-packages/qiime2/sdk/", line 390, in callable_executor
output_views = self._callable(**view_args)
File "/opt/conda/envs/qiime2-2020.8/lib/python3.6/site-packages/q2_fragment_insertion/", line 77, in sepp
File "/opt/conda/envs/qiime2-2020.8/lib/python3.6/site-packages/q2_fragment_insertion/", line 53, in _run, check=True, cwd=cwd)
File "/opt/conda/envs/qiime2-2020.8/lib/python3.6/", line 438, in run
output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['', '/tmp/qiime2-archive-kwte99ss/3407a126-de9d-4a83-9f9f-93c4b6bd8503/data/dna-sequences.fasta', 'q2-fragment-insertion', '-x', '14', '-A', '1000', '-P', '5000', '-a', '/tmp/qiime2-archive-g8_r8d_m/e44b5e78-31e5-4a0f-9041-494bc3ca2df2/data/aligned-dna-sequences.fasta', '-t', '/tmp/qiime2-archive-g8_r8d_m/e44b5e78-31e5-4a0f-9041-494bc3ca2df2/data/tree.nwk', '-r', '/tmp/qiime2-archive-g8_r8d_m/e44b5e78-31e5-4a0f-9041-494bc3ca2df2/data/raxml-info.txt']' returned non-zero exit status 1.
Would appreciate any help.
Thank you!

Hi @rosave - looks like this is a duplicate post from your original submission. I am going to go ahead and close this one out - but one of our moderators will follow up with you on your original post with assistance!

Cheers :lizard: