# Error using MetONTIIME - Missing fromPath parameter

**URL:** https://forum.qiime2.org/t/error-using-metontiime-missing-frompath-parameter/28345
**Category:** Other Bioinformatics Tools
**Tags:** nanopore
**Created:** [November 20, 2023, 6:58am UTC](https://forum.qiime2.org/t/error-using-metontiime-missing-frompath-parameter/28345 "2023-11-20T06:58:14Z")
**Posts on this page:** 4
**Page:** 1

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### Author: ![Rebekah\_Henry1](https://forum.qiime2.org/user_avatar/forum.qiime2.org/rebekah_henry1/32/17176_2.png) [@Rebekah\_Henry1](https://forum.qiime2.org/u/Rebekah_Henry1)
#### Post date: [November 20, 2023, 6:58am UTC](https://forum.qiime2.org/t/error-using-metontiime-missing-frompath-parameter/28345/1 "2023-11-20T06:58:14Z")

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Hi All,

I have used QIIME2 alot but generally with Illumina data. I am not attempting to use MetONTIIME for nanopore through nextflow, but keep hitting the same error.

Launching `metontiime2.nf` [wise\_swartz] DSL2 - revision: 3a67d1d68a  
Missing `fromPath` parameter

I have had a look through both the .conf and .nf files (attached below) and can't see what I have done wrong in the configurations. Could someone who has done this before have a look and tell me where I am going wrong.

Kind regards  
[metontiime2\_conf.txt](https://forum.qiime2.org/uploads/short-url/3xyf5OYKSNgzbwcr9dwAr4Tjrsj.txt) (9.8 KB)  
[metontiime2\_nf.txt](https://forum.qiime2.org/uploads/short-url/jwF2x9okDZjNSV95GPToxn8aArO.txt) (26.8 KB)

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### Author: ![gregcaporaso](https://forum.qiime2.org/user_avatar/forum.qiime2.org/gregcaporaso/32/17769_2.png) [@gregcaporaso](https://forum.qiime2.org/u/gregcaporaso)
#### Post date: [November 27, 2023, 8:42pm UTC](https://forum.qiime2.org/t/error-using-metontiime-missing-frompath-parameter/28345/5 "2023-11-27T20:42:47Z")

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Hi @Rebekah_Henry1,  
Welcome to the QIIME 2 Forum! None of our developers have experience with MetONTIIME (it's not developed by us), but I just shared your post with the MetONTIIME developer in the hope that they can answer your question.

Thanks for your interest in QIIME 2!

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### Author: ![MaestSi](https://forum.qiime2.org/user_avatar/forum.qiime2.org/maestsi/32/12152_2.png) [@MaestSi](https://forum.qiime2.org/u/MaestSi)
#### Post date: [November 28, 2023, 11:06am UTC](https://forum.qiime2.org/t/error-using-metontiime-missing-frompath-parameter/28345/6 "2023-11-28T11:06:51Z")

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Dear @Rebekah_Henry1,  
I think there are two main issues in the config file. First, since your files are in `/fs03` folder, you should make it accessible to either docker or singularity containers.  
To do so, if you are using singularity profile, you should edit line 74 from:  
`containerOptions = '--bind /home/:/home'`  
to:  
`containerOptions = '--bind /home/:/home --bind /fs03:/fs03'`

While, if you are using docker profile you should edit line 171 from:  
`containerOptions = '-v /home/:/home'`  
to  
`containerOptions = '-v /home/:/home -v /fs03:/fs03'`

Second, I saw you probably already have qza files for both sequences and taxonomy. If that is the case, they should be put in `/fs03/pv54/Beks/Raw-Reads/nanopore_silvan/run1_metontiime/importDb` folder with files names specified by `dbSequencesQza` and `dbTaxonomyQza` parameters, respectively, and the `importDb` process (line 53) should be set to false with:  
`importDb = false`  
While, if you would like to start from fasta and tsv files, it's ok to leave the `importDb` process set to `true` and you should specify the full path to those files as values for `dbSequencesFasta` and `dbTaxonomyTsv` parameters.  
I hope this solves your issue, but if you have any further questions, please open an issue [in the GitHub repository](https://github.com/MaestSi/MetONTIIME).  
P.s.: I would suggest first testing the pipeline configuration with the demo dataset uploaded in the repo.  
Best,  
SM

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [December 29, 2023, 5:07pm UTC](https://forum.qiime2.org/t/error-using-metontiime-missing-frompath-parameter/28345/7 "2023-12-29T17:07:30Z")

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