Hi. I am trying to run Picrust2 using Qiime2-2019.4. I am using the following command (which is exactly what is shown in the tutorial):
qiime picrust2 full-pipeline --i-table table.qza
I then get the following error:
(1/1) Invalid value for “–i-seq”: ‘rep_seqs.qza’ is not a QIIME 2 Artifact
I am using files created in qiime2 with dada2.
Thanks for any advice you may have.
Could you please run
qiime tools validate on your rep-seqs artifact and post the results please?
Here’s the output when I run qiime tools validate rep-seqs.qza:
Result rep-seqs.qza appears to be valid at level=max.
We have seen this error when q2cli is unable to find the file specified — any chance something was wrong with the path in your picrust2 invocation? Did you run the validation from the same directory as the picrust2 command?
Thank you @thermokarst…I see my issue now…I had put ‘rep_seq.qza’ instead of ‘rep-seq.qza’. It’s working now!
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