# Error running DADA2 in 454 data

**URL:** https://forum.qiime2.org/t/error-running-dada2-in-454-data/8051
**Category:** Technical Support
**Created:** [January 29, 2019, 12:41am UTC](https://forum.qiime2.org/t/error-running-dada2-in-454-data/8051 "2019-01-29T00:41:55Z")
**Posts on this page:** 1
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### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [January 29, 2019, 2:39pm UTC](https://forum.qiime2.org/t/error-running-dada2-in-454-data/8051/2 "2019-01-29T14:39:15Z")

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> [@jbethany](#):
>
> Error in err[c(1, 6, 11, 16),] \<- 1 :  
> incorrect number of subscripts on matrix

I found a similar error on the DADA2 error tracker:

[https://github.com/benjjneb/dada2/issues/614](https://github.com/benjjneb/dada2/issues/614)

Maybe you can try some of the checks suggested in that thread? You will already have a copy of DADA2 (and R) on hand in your QIIME 2 env. FWIW, in the linked issue it sounds like the reason this error happened is because all of the reads in the data were unique - no groupings of reads were identified.

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