ERROR RAISED DURING STEP: Assign taxonomy [QIIME 1]

Hi

I have installed QIIME and I am going over ‘‘454 Overview Tutorial: de novo OTU picking and diversity analyses using 454 data’’ trying to apply each step using data provided in the QIIME website.

I have encountered an error trying to perform ‘‘De novo OTU picking’’ section of the tutorial using pick_de_novo_otus.py. I also looked at my directory and I noticed that the file containing Taxonomic Assignment has not been created.

I have put the log file here and I would appreciate if anyone could help.
log_20200414103428.txt (14.2 KB)

Sorry I am a newbie in Qiime.

1 Like

Welcome to the forums!

You have come to the right place! We are here to help :handshake:


That tutorial is pretty old (as it still uses Qiime 1, which we don’t officially support anymore.)

You can use Qiime 1, if you want, but if you are getting started for the first time, I would suggest using Qiime 2. It’s faster :dash:, supports cutting-edge methods :rocket:, and is easier to use :hugs: (in my opinion).

Get Qiime 2 here, then try importing your data as shown over here.

(I’m willing to answer questions about Qiime 1, but this forum focuses on supporting the newest version of Qiime, which is part of the reason I’m suggesting the upgrade!)

Colin

3 Likes

Thank you so much for your suggestion. I just started using Qiime 2.

3 Likes