# Error phylogen (fasttree)

**URL:** https://forum.qiime2.org/t/error-phylogen-fasttree/4227
**Category:** User Support
**Tags:** taxonomy, phylogeny, tree
**Created:** [May 21, 2018, 5:23pm UTC](https://forum.qiime2.org/t/error-phylogen-fasttree/4227 "2018-05-21T17:23:35Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![Nasute](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nasute/32/3846_2.png) [@Nasute](https://forum.qiime2.org/u/Nasute)
#### Post date: [May 21, 2018, 5:23pm UTC](https://forum.qiime2.org/t/error-phylogen-fasttree/4227/1 "2018-05-21T17:23:35Z")

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Hi Everyone!

Hello, I am all experiencing a problem with the creation of a phylogenetic tree using the "fasttree" program since I apparently have an (excess of arguments) however, I am using the parameters that come by default in fasttree: construct and phylogenetic tree with Fast tree  
This is my input file  
qiime phylogeny fasttree   
--i-alignment aligned-rep-seqs.qza   
--p-n-threads 1  
--o-tree unrooted-tree.qza  
--output-dir \home\julissa\phylogeny  
--verbose  
attached error screenshot

 ![Captura%20de%20pantalla%20de%202018-05-21%2012-09-40](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/5/5f4ba98427ddf3b537fe701afb95ea9ed1c7ec46.png)

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### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [May 21, 2018, 11:04pm UTC](https://forum.qiime2.org/t/error-phylogen-fasttree/4227/2 "2018-05-21T23:04:11Z")

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Hello Julissa,

That screenshot is perfect. I think I have found the problem.

It looks you have `\` back slashes `\` in your command. Take these out, the try running the command again.

Colin

P.S. I should explain that back slashes separate a command between multiple lines. Like this

```auto
command --i file --o files --note "This works"

command --i file \
--o files \
--note "This works, and it's on three lines"

command --i file \ --o files \ --note "this does NOT work"

```

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [June 22, 2018, 5:10am UTC](https://forum.qiime2.org/t/error-phylogen-fasttree/4227/3 "2018-06-22T05:10:50Z")

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